Recombinant Carboxypeptidase B

Animal Origin Free, No Virus Contaminant

Catalog Number:RCPB01

CAS: 9025-24-5
EC: 3.4.17.2

Source: Expressed in E. Coli.

DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 34, 500 daltons, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.

ADVANTAGES

Animal origin free:YaxinBio recombinant carboxypeptidase B is animal components free, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B.

Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3) Less than 10ppm of recombinant trypsin.

MAIN FEATURES

Source	E. Coli
Format	White or white-like or yellowish lyophilized
Additives	Tris salts, NaCl salts and carbohydrates
Protein content	35% ～ 60%
Specific activity	≥170 units/mg pro.
Purity	Single main band on SDS-PAGE
M.W.	35kD
Contaminant activity	Less than 10 ppm of recombinant trypsin.

UNIT DEFINITION：One Unit of carboxypeptidase B activity hydrolyzes one micromole of

hippuryl-L-arginine per minute at 25℃, pH 7.65.

RECOMMEND USAGE

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.0-9.0.

STORAGE INSTRUCTIONS

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.