Recombinant Protein A

No antibody contaminant. No antibody affinity column purification.

Catalog Number:RSPA05

CAS:91932-65-9

Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli, .

APPLICATION:

The recombinant protein A is a genetically engineering protein containing IgG-binding domains. Recombinant protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.

Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.

PRODUCT SPECIFICATION

Test/Method	Specification
Bioburden	No organisms detected.
12% SDS-PAGE	Single major band
Coomassie Stain	25kD
UV Spectrum	E275/250 ≥1.2, E0.1% at 275nm=0.17-0.23
UV spectrometry	No DNA or RNA detected
Triton Content	No Triton contaminant.
Format	Lyophilized
pH stable range	pH 1-13

STORAGE INSTRUCTIONS

Recombinant protein A lyophilized should be stored under 2℃-8℃ in sealed container.It is stable within 24 months.

USAGE:

For Research or Manufacturing Purpose Only. Not for Human.